ELK1411-48T, Rat CNTF(Ciliary Neurotrophic Factor) ELISA Kit, 48T

ELK1411-48T, Rat CNTF(Ciliary Neurotrophic Factor) ELISA Kit, 48T

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ELK1411-96T, Rat CNTF(Ciliary Neurotrophic Factor) ELISA Kit, 96T

2.963,10 RON

Rat CNTF(Ciliary Neurotrophic Factor) ELISA Kit

SKU
ELK1411-96T

Alternative Names: HCNTF

Species: Rat

Assay Type: Sandwich

Sensitivity: 16 pg/mL

Standard: 2500 pg/mL

Detection range: 39.07-2500 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine;Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CNTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CNTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CNTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CNTF in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: HCNTF

Species: Rat

Assay Type: Sandwich

Sensitivity: 16 pg/mL

Standard: 2500 pg/mL

Detection range: 39.07-2500 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine;Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CNTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CNTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CNTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CNTF in the samples is then determined by comparing the OD of the samples to the standard curve.