ELK1227-96T, Human CASP8(Caspase 8) ELISA Kit, 96T

Alternative Names: CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

Species: Human

Assay Type: Sandwich

Sensitivity: 0.244 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CASP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CASP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CASP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CASP8 in the samples is then determined by comparing the OD of the samples to the standard curve.