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ELK1198-96T, Human CA19-9(Carbohydrate Antigen 19-9) ELISA Kit, 96T
2.475,20 RON
Human CA19-9(Carbohydrate Antigen 19-9) ELISA Kit
SKU
ELK1198-96T
Alternative Names: sialylated Lewis(a)Antigen; Cancer Antigen 19-9
Species: Human
Assay Type: Sandwich
Sensitivity: 0.28 U/mL
Standard: 50 U/mL
Detection range: 0.79-50 U/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 3.5h
Research Area: Tumor immunity;Hepatology;Gastroenterology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: sialylated Lewis(a)Antigen; Cancer Antigen 19-9 Species: Human Assay Type: Sandwich Sensitivity: 0.28 U/mL Standard: 50 U/mL Detection range: 0.79-50 U/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay length: 3.5h Research Area: Tumor immunity;Hepatology;Gastroenterology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
