ELK1164-96T, Rabbit TNFa(Tumor Necrosis Factor Alpha) ELISA Kit, 96T

Alternative Names: TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 1.17 pg/mL

Standard: 200 pg/mL

Detection range: 3.13-200 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.