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ELK1163-96T, Human TSH(Thyroid Stimulating Hormone) ELISA Kit, 96T
2.475,20 RON
Human TSH(Thyroid Stimulating Hormone) ELISA Kit
SKU
ELK1163-96T
Alternative Names: Thyrotropin
Species: Human
Assay Type: Sandwich
Sensitivity: 0.11 uIU/mL
Standard: 12 uIU/mL
Detection range: 0.19-12 uIU/mL
Sample type: Serum
Assay length: 3.5h
Research Area: Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSH in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Thyrotropin Species: Human Assay Type: Sandwich Sensitivity: 0.11 uIU/mL Standard: 12 uIU/mL Detection range: 0.19-12 uIU/mL Sample type: Serum Assay length: 3.5h Research Area: Endocrinology;Hormone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSH in the samples is then determined by comparing the OD of the samples to the standard curve. |
