ELK1140-48T, Mouse IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit, 48T

Alternative Names: BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.37 ng/mL

Standard: 60 ng/mL

Detection range: 0.94-60 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.