ELK1093-48T, Human HB(Hemoglobin) ELISA Kit, 48T

ELK1093-48T, Human HB(Hemoglobin) ELISA Kit, 48T

ELK1094-48T, Human MPIF2(Myeloid Progenitor Inhibitory Factor 2) ELISA Kit, 48T

ELK1094-48T, Human MPIF2(Myeloid Progenitor Inhibitory Factor 2) ELISA Kit, 48T

ELK1093-96T, Human HB(Hemoglobin) ELISA Kit, 96T

2.475,20 RON

Human HB(Hemoglobin) ELISA Kit

SKU
ELK1093-96T

Alternative Names: Hgb; Haemoglobin; Heterotetramer(αβ)2

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.62 µg/mL

Standard: 500 µg/mL

Detection range: 7.82-500 µg/mL

Sample type: serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HB in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Hgb; Haemoglobin; Heterotetramer(αβ)2

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.62 µg/mL

Standard: 500 µg/mL

Detection range: 7.82-500 µg/mL

Sample type: serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HB in the samples is then determined by comparing the OD of the samples to the standard curve.