ELK1081-96T, Human GC(Glucagon) ELISA Kit, 96T

Alternative Names: GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 7.54 pg/mL

Standard: 1500 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Endocrinology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GC in the samples is then determined by comparing the OD of the samples to the standard curve.