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ELK1060-96T, Human REN(Renin) ELISA Kit, 96T
2.475,20 RON
Human REN(Renin) ELISA Kit
SKU
ELK1060-96T
Alternative Names: angiotensinogenase
Species: Human
Assay Type: Sandwich
Sensitivity: 11.6 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;Kidney biomarker;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REN in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: angiotensinogenase Species: Human Assay Type: Sandwich Sensitivity: 11.6 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Endocrinology;Cardiovascular biology;Kidney biomarker;Hormone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REN in the samples is then determined by comparing the OD of the samples to the standard curve. |
