ELK0995-48T, Chicken IL1α(Interleukin 1 Alpha) ELISA Kit, 48T

Alternative Names: IL1 Alpha; IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1

Species: Chicken

Assay Type: Sandwich

Sensitivity: 2.7 pg/mL

Standard: 500 pg/mL

Detection range: 7.82-500 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Apoptosis;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IL1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IL1α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IL1α in the samples is then determined by comparing the OD of the samples to the standard curve.