ELK0962-96T, Rat p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit, 96T

Alternative Names: p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 1.24 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.