ELK0946-48T, Mouse JNK(c-Jun N-terminal kinases) ELISA Kit, 48T

ELK0946-48T, Mouse JNK(c-Jun N-terminal kinases) ELISA Kit, 48T

ELK0947-48T, Rat NLRP6(NACHT, LRR and PYD domains-containing protein 6) ELISA Kit, 48T

ELK0947-48T, Rat NLRP6(NACHT, LRR and PYD domains-containing protein 6) ELISA Kit, 48T

ELK0946-96T, Mouse JNK(c-Jun N-terminal kinases) ELISA Kit, 96T

2.963,10 RON

Mouse JNK(c-Jun N-terminal kinases) ELISA Kit

SKU
ELK0946-96T

Alternative Names: MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; c-Jun N-terminal kinase JNK

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.14 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse JNK in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; c-Jun N-terminal kinase JNK

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.14 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse JNK in the samples is then determined by comparing the OD of the samples to the standard curve.