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ELK0915-48T, Human T3(Triiodothyronine) ELISA Kit, 48T
1.814,75 RON
Human T3(Triiodothyronine) ELISA Kit
SKU
ELK0915-48T
Alternative Names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.073 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human T3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human T3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin Species: Human Assay Type: Competitive Inhibition Sensitivity: 0.073 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 2.5h Research Area: Endocrinology;Autoimmunity; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human T3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human T3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
