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ELK0913-96T, Human LTB4(Leukotriene B4) ELISA Kit, 96T
2.475,20 RON
Human LTB4(Leukotriene B4) ELISA Kit
SKU
ELK0913-96T
Alternative Names: LT-B4
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.19 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human LTB4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LTB4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTB4 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: LT-B4 Species: Human Assay Type: Competitive Inhibition Sensitivity: 0.19 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human LTB4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LTB4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTB4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
