ELK0886-48T, Mouse PADI6(Peptidyl Arginine Deiminase Type VI) ELISA Kit, 48T

ELK0886-48T, Mouse PADI6(Peptidyl Arginine Deiminase Type VI) ELISA Kit, 48T

ELK0887-48T, Mouse PADI4(Peptidyl Arginine Deiminase Type IV) ELISA Kit, 48T

ELK0887-48T, Mouse PADI4(Peptidyl Arginine Deiminase Type IV) ELISA Kit, 48T

ELK0886-96T, Mouse PADI6(Peptidyl Arginine Deiminase Type VI) ELISA Kit, 96T

2.963,10 RON

Mouse PADI6(Peptidyl Arginine Deiminase Type VI) ELISA Kit

SKU
ELK0886-96T

Alternative Names: Peptidylarginine deiminase VI; Peptidyl arginine deiminase-like protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.071 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PADI6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PADI6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PADI6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PADI6 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Peptidylarginine deiminase VI; Peptidyl arginine deiminase-like protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.071 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PADI6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PADI6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PADI6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PADI6 in the samples is then determined by comparing the OD of the samples to the standard curve.