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ELK0865-96T, Human Anti-FOLR(folate receptor autoantibodies IgG) ELISA Kit, 96T

2.475,20 RON

Human Anti-FOLR(folate receptor autoantibodies IgG) ELISA Kit

SKU
ELK0865-96T

Alternative Names: ANTI-FOLR1

Species: Human

Assay Type: Sandwich

Sensitivity: 3.2 ng/mL

Standard: 500 ng/mL

Detection range: 7.82-500 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-FOLR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-FOLR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-FOLR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-FOLR in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: ANTI-FOLR1

Species: Human

Assay Type: Sandwich

Sensitivity: 3.2 ng/mL

Standard: 500 ng/mL

Detection range: 7.82-500 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-FOLR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-FOLR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-FOLR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-FOLR in the samples is then determined by comparing the OD of the samples to the standard curve.