ELK085ES-96T, EasyStep Human IL8(Interleukin 8) ELISA Kit, 96T

Alternative Names: IL8

Species: Human

Assay Type: Sandwich

Sensitivity: 3.44 pg/ml

Standard:

Detection range: 6.25 pg/ml - 400 pg/ml

Sample type: cellular supernatant

Assay length: 1.5h

Research Area:

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA. After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.