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ELK0843-48T, Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit, 48T
1.814,75 RON
Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
SKU
ELK0843-48T
Alternative Names: 25 Hydroxy Vitamin D;
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 0.91 ng/mL
Standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 25 Hydroxy Vitamin D; Species: Rat Assay Type: Competitive Inhibition Sensitivity: 0.91 ng/mL Standard: 200 ng/mL Detection range: 3.13-200 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve. |
