ELK0840-48T, Pig CRH(Corticotropin Releasing Hormone) ELISA Kit, 48T

ELK0840-48T, Pig CRH(Corticotropin Releasing Hormone) ELISA Kit, 48T

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ELK0840-96T, Pig CRH(Corticotropin Releasing Hormone) ELISA Kit, 96T

2.963,10 RON

Pig CRH(Corticotropin Releasing Hormone) ELISA Kit

SKU
ELK0840-96T

Alternative Names: CRF; Corticotropin Releasing Factor; Corticoliberin

Species: Pig

Assay Type: Competitive Inhibition

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CRF; Corticotropin Releasing Factor; Corticoliberin

Species: Pig

Assay Type: Competitive Inhibition

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CRH in the samples is then determined by comparing the OD of the samples to the standard curve.