ELK0830-96T, Mouse TXLNa(Taxilin Alpha) ELISA Kit, 96T

Alternative Names: IL14; TXLN; HMW-BCGF; High Molecular Weight B-Cell Growth Factor; Interleukin 14

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length: 3.5h

Research Area: Metabolic pathway;Immune molecule;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TXLNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TXLNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TXLNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TXLNa in the samples is then determined by comparing the OD of the samples to the standard curve.