ELK0829-96T, Mouse CD200R2(CD200 Receptor 2) ELISA Kit, 96T

Alternative Names: CD200R1L; CD200RLa; HuCD200R2; Cell surface glycoprotein CD200 receptor 2; CD200 cell surface glycoprotein receptor-like a; Cell surface glycoprotein OX2 receptor 2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.067 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: CD & Adhesion molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD200R2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD200R2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD200R2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD200R2 in the samples is then determined by comparing the OD of the samples to the standard curve.