ELK0812-48T, Rat Glu(Glutamic Acid) ELISA Kit, 48T

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ELK0812-96T, Rat Glu(Glutamic Acid) ELISA Kit, 96T

2.475,20 RON

Rat Glu(Glutamic Acid) ELISA Kit

SKU
ELK0812-96T

Alternative Names: Glutamate

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 48.2 ng/mL

Standard: 10000 ng/mL

Detection range: 156.25-10000 ng/mL

Sample type: biological fluids

Assay length: 2h

Research Area: Enzyme & Kinase;Endocrinology;Neuro science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Glu protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Glu. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Glu in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Glutamate

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 48.2 ng/mL

Standard: 10000 ng/mL

Detection range: 156.25-10000 ng/mL

Sample type: biological fluids

Assay length: 2h

Research Area: Enzyme & Kinase;Endocrinology;Neuro science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Glu protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Glu. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Glu in the samples is then determined by comparing the OD of the samples to the standard curve.