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ELK0811-96T, Human VD3(Vitamin D3) ELISA Kit, 96T
2.475,20 RON
Human VD3(Vitamin D3) ELISA Kit
SKU
ELK0811-96T
Alternative Names: Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 2.14 ng/mL
Standard: 400 ng/mL
Detection range: 6.25-400 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol Species: Human Assay Type: Competitive Inhibition Sensitivity: 2.14 ng/mL Standard: 400 ng/mL Detection range: 6.25-400 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
