ELK0808-96T, Human Acetyl-H3(Acetyl Histone H3) ELISA Kit, 96T

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ELK0809-96T, Human fT4(Free Thyroxine) ELISA Kit, 96T

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ELK0809-48T, Human fT4(Free Thyroxine) ELISA Kit, 48T

1.814,75 RON

Human fT4(Free Thyroxine) ELISA Kit

SKU
ELK0809-48T

Alternative Names: FTI

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.48 pg/mL

Standard: 80 pg/mL

Detection range: 1.25-80 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human fT4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human fT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: FTI

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.48 pg/mL

Standard: 80 pg/mL

Detection range: 1.25-80 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human fT4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human fT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.