ELK0803-48T, Mouse CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit, 48T

Alternative Names: CREB; active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 1.17 pg/mL

Standard: 200 pg/mL

Detection range: 3.13-200 pg/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Epigenetics and Nuclear Signaling; Immunology; Neuroscience

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CREB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CREB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CREB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CREB in the samples is then determined by comparing the OD of the samples to the standard curve.