ELK0800-96T, Rat NBC4(Electrogenic Sodium Bicarbonate Cotransporter 4) ELISA Kit, 96T

Alternative Names: SLC4A5; NBCe2; Solute Carrier Family 4 Member 5,Sodium Bicarbonate Cotransporter

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.31 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NBC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NBC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NBC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NBC4 in the samples is then determined by comparing the OD of the samples to the standard curve.