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ELK0787-96T, Rat Bb(Bilirubin) ELISA Kit, 96T
2.475,20 RON
Rat Bb(Bilirubin) ELISA Kit
SKU
ELK0787-96T
Alternative Names: Hematoidin
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 0.45 µg/mL
Standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Liver function
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Bb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bb in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Hematoidin Species: Rat Assay Type: Competitive Inhibition Sensitivity: 0.45 µg/mL Standard: 100 µg/mL Detection range: 1.57-100 µg/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Liver function Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Bb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bb in the samples is then determined by comparing the OD of the samples to the standard curve. |
