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ELK0778-48T, Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit, 48T
2.142,00 RON
Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit
SKU
ELK0778-48T
Alternative Names: 2,3DPG
Species: Human
Assay Type: Sandwich
Sensitivity: 0.14 µmol/mL
Standard: 20 µmol/mL
Detection range: 0.32-20 µmol/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 2,3DPG Species: Human Assay Type: Sandwich Sensitivity: 0.14 µmol/mL Standard: 20 µmol/mL Detection range: 0.32-20 µmol/mL Sample type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve. |
