ELK0772-48T, PROA (Protein A) ELISA Kit, 48T

Alternative Names: Staphylococcus aureus protein A (SpA)

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 18.6 pg/mL

Standard: 3000 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: serum, plasma, other biological fluids

Assay length: 2.5h

Research Area: Tumor immunity;Endocrinology;Cardiovascular biology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PROA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PROA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PROA in the samples is then determined by comparing the OD of the samples to the standard curve.