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ELK0764-48T, Human Infliximab ELISA Kit, 48T
2.142,00 RON
Human Infliximab ELISA Kit
SKU
ELK0764-48T
Alternative Names: infliximab
Species: Human
Assay Type: Sandwich
Sensitivity: 1.81 ng/mL
Standard: 300 ng/mL
Detection range: 4.69-300 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Immunology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Infliximab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Infliximab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Infliximab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Infliximab in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: infliximab Species: Human Assay Type: Sandwich Sensitivity: 1.81 ng/mL Standard: 300 ng/mL Detection range: 4.69-300 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Immunology Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Infliximab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Infliximab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Infliximab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Infliximab in the samples is then determined by comparing the OD of the samples to the standard curve. |
