ELK0755-96T, Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit, 96T

ELK0755-96T, Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit, 96T

ELK0756-96T, Rat PREG(Pregnenolone) ELISA Kit, 96T

ELK0756-96T, Rat PREG(Pregnenolone) ELISA Kit, 96T

ELK0756-48T, Rat PREG(Pregnenolone) ELISA Kit, 48T

1.814,75 RON

Rat PREG(Pregnenolone) ELISA Kit

SKU
ELK0756-48T

Alternative Names: Pregnenolone

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 6.81 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2.5h

Research Area: Reproductive science

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PREG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PREG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PREG in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Pregnenolone

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 6.81 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2.5h

Research Area: Reproductive science

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PREG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PREG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PREG in the samples is then determined by comparing the OD of the samples to the standard curve.