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ELK0754-96T, Rat cAMP(Cyclic Adenosine Monophosphate) ELISA Kit, 96T
3.379,60 RON
Rat cAMP(Cyclic Adenosine Monophosphate) ELISA Kit
SKU
ELK0754-96T
Alternative Names: c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 143.2 pg/mL
Standard: 20000 pg/mL
Detection range: 312.5-20000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Signal transduction;Metabolic pathway;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat cAMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.840,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate Species: Rat Assay Type: Competitive Inhibition Sensitivity: 143.2 pg/mL Standard: 20000 pg/mL Detection range: 312.5-20000 pg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2.5h Research Area: Signal transduction;Metabolic pathway; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat cAMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat cAMP in the samples is then determined by comparing the OD of the samples to the standard curve. |
