ELK0752-96T, Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit, 96T

Alternative Names: Anti-Tissue Transglutaminase IgA

Species: Human

Assay Type: Sandwich

Sensitivity: 1.47 IU/mL

Standard: 300 IU/mL

Detection range: 4.69-300 IU/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.