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ELK0731-96T, Human JAK2(Tyrosine-Protein Kinase JAK2) ELISA Kit, 96T
2.963,10 RON
Human JAK2(Tyrosine-Protein Kinase JAK2) ELISA Kit
SKU
ELK0731-96T
Alternative Names: THCYT3; JTK10
Species: Human
Assay Type: Sandwich
Sensitivity: 41.2 pg/mL
Standard: 8000 pg/mL
Detection range: 125-8000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human JAK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human JAK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human JAK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human JAK2 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: THCYT3; JTK10 Species: Human Assay Type: Sandwich Sensitivity: 41.2 pg/mL Standard: 8000 pg/mL Detection range: 125-8000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human JAK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human JAK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human JAK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human JAK2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
