ELK0702-96T, Human MAPK(Mitogen Activated Protein Kinase) ELISA Kit, 96T

ELK0702-96T, Human MAPK(Mitogen Activated Protein Kinase) ELISA Kit, 96T

ELK0703-96T, Human MAS1(Proto-oncogene Mas) ELISA Kit, 96T

ELK0703-96T, Human MAS1(Proto-oncogene Mas) ELISA Kit, 96T

ELK0703-48T, Human MAS1(Proto-oncogene Mas) ELISA Kit, 48T

2.142,00 RON

Human MAS1(Proto-oncogene Mas) ELISA Kit

SKU
ELK0703-48T

Alternative Names: MAS; MGRA

Species: Human

Assay Type: Sandwich

Sensitivity: 0.12 ng/mL

Standard: 20 ng/ml

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAS1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: MAS; MGRA

Species: Human

Assay Type: Sandwich

Sensitivity: 0.12 ng/mL

Standard: 20 ng/ml

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAS1 in the samples is then determined by comparing the OD of the samples to the standard curve.