ELK0694-48T, Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit, 48T

Alternative Names: 25 Hydroxy Vitamin D;

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 1.41 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2.5h

Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.