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ELK0685-96T, Mouse KL-6(Krebs Von den Lungen-6) ELISA Kit, 96T
2.963,10 RON
Mouse KL-6(Krebs Von den Lungen-6) ELISA Kit
SKU
ELK0685-96T
Alternative Names: KL-6; Mucin-1; MUC1
Species: Mouse
Assay Type: Sandwich
Sensitivity: 32.1 pg/mL
Standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Interstitial lung disease
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: KL-6; Mucin-1; MUC1 Species: Mouse Assay Type: Sandwich Sensitivity: 32.1 pg/mL Standard: 5000 pg/mL Detection range: 78.13-5000 pg/mL Sample type: serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Interstitial lung disease Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
