ELK0670-48T, Mouse GPX7(Glutathione Peroxidase 7) ELISA Kit, 48T

ELK0670-48T, Mouse GPX7(Glutathione Peroxidase 7) ELISA Kit, 48T

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ELK0670-96T, Mouse GPX7(Glutathione Peroxidase 7) ELISA Kit, 96T

2.963,10 RON

Mouse GPX7(Glutathione Peroxidase 7) ELISA Kit

SKU
ELK0670-96T

Alternative Names: GPX6; 3110050F08Rik; AI327032

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.069 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GPX7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GPX7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GPX7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GPX7 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: GPX6; 3110050F08Rik; AI327032

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.069 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GPX7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GPX7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GPX7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GPX7 in the samples is then determined by comparing the OD of the samples to the standard curve.