ELK0622-96T, Dog PG(Progesterone) ELISA Kit, 96T

Alternative Names: P4; Pregn-4-Ene-3,20-Dione

Species: Dog

Assay Type: Competitive Inhibition

Sensitivity: 0.31 ng/mL

Standard: 50 ng/mL

Detection range: 0.78-50 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2.5h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog PG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog PG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog PG in the samples is then determined by comparing the OD of the samples to the standard curve.