EasyStep Human PGI(Pepsinogen I) ELISA Kit
Alternative Names: PGI
Species: Human
Assay Type: Sandwich
Sensitivity: 18 pg/ml
Standard: 36.06 ng/ml
Detection range: 0.31 ng/ml - 20 ng/ml
Sample type: Serum and plasma
Assay length: 1.5h
Research Area:
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA. After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 3.140,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: PGI Species: Human Assay Type: Sandwich Sensitivity: 18 pg/ml Standard: 36.06 ng/ml Detection range: 0.31 ng/ml - 20 ng/ml Sample type: Serum and plasma Assay length: 1.5h Research Area: Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA. After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve. |