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ELK0613-48T, Cattle COL10(Collagen Type X) ELISA Kit, 48T
2.142,00 RON
Cattle COL10(Collagen Type X) ELISA Kit
SKU
ELK0613-48T
Alternative Names: COL10A1; COL10-A1; Schmid Metaphyseal Chondrodysplasia; Collagen Alpha-1(X)chain
Species: Cattle
Assay Type: Sandwich
Sensitivity: 0.81 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle COL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle COL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle COL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle COL10 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: COL10A1; COL10-A1; Schmid Metaphyseal Chondrodysplasia; Collagen Alpha-1(X)chain Species: Cattle Assay Type: Sandwich Sensitivity: 0.81 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle COL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle COL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle COL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle COL10 in the samples is then determined by comparing the OD of the samples to the standard curve. |
