Rat GA(Glycated Albumin) ELISA Kit
Alternative Names: Glycated Albumin,GA
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 5.16 pmol/mL
Standard: 1000 pmol/mL
Detection range: 15.63-1000 pmol/mL
Sample type: serum, plasma
Assay length: 2.5h
Research Area: diabetic nephropathy
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GA in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.490,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: Glycated Albumin,GA Species: Rat Assay Type: Competitive Inhibition Sensitivity: 5.16 pmol/mL Standard: 1000 pmol/mL Detection range: 15.63-1000 pmol/mL Sample type: serum, plasma Assay length: 2.5h Research Area: diabetic nephropathy Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GA in the samples is then determined by comparing the OD of the samples to the standard curve. |