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ELK0603-48T, Cattle GnRH(Gonadotropin Releasing Hormone) ELISA Kit, 48T
2.142,00 RON
Cattle GnRH(Gonadotropin Releasing Hormone) ELISA Kit
SKU
ELK0603-48T
Alternative Names: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
Species: Cattle
Assay Type: Competitive Inhibition
Sensitivity: 23.1 pg/mL
Standard: 4000 pg/mL
Detection range: 62.50-4000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1 Species: Cattle Assay Type: Competitive Inhibition Sensitivity: 23.1 pg/mL Standard: 4000 pg/mL Detection range: 62.50-4000 pg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2.5h Research Area: Endocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle GnRH in the samples is then determined by comparing the OD of the samples to the standard curve. |
