ELK0580-48T, Zebrafish gABA(Gamma-Aminobutyric Acid) ELISA Kit, 48T

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ELK0580-96T, Zebrafish gABA(Gamma-Aminobutyric Acid) ELISA Kit, 96T

2.475,20 RON

Zebrafish gABA(Gamma-Aminobutyric Acid) ELISA Kit

SKU
ELK0580-96T

Alternative Names: 4-Aminobutyric Acid

Species: Zebrafish

Assay Type: Competitive Inhibition

Sensitivity: 16.1 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2.5h

Research Area: Signal transduction;Cardiovascular biology;Neuro science;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish gABA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish gABA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: 4-Aminobutyric Acid

Species: Zebrafish

Assay Type: Competitive Inhibition

Sensitivity: 16.1 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2.5h

Research Area: Signal transduction;Cardiovascular biology;Neuro science;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish gABA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish gABA in the samples is then determined by comparing the OD of the samples to the standard curve.