ELK057ES-96T, EasyStep Human cTnI(Cardiac Troponin I) ELISA Kit, 96T

Alternative Names: TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Cardiac troponin I; Troponin I

Species: Human

Assay Type: Sandwich

Sensitivity: 19.6 pg/mL

Standard: 25 ng/mL

Detection range: 0.39-25 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human cTnI, and the Human cTnI standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human cTnI. After TMB substrate solution is added, only those wells that contain Human cTnI and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.