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ELK0571-48T, Human 6-K-PGF1α(6-keto-PGF1 alpha) ELISA Kit, 48T

2.142,00 RON

Human 6-K-PGF1α(6-keto-PGF1 alpha) ELISA Kit

SKU
ELK0571-48T

Alternative Names: 6-keto-PGF1 alpha; 6ketoPGF1α

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 5.7 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2.5h

Research Area: Neuro science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 6-K-PGF1α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 6-K-PGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 6-K-PGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: 6-keto-PGF1 alpha; 6ketoPGF1α

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 5.7 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2.5h

Research Area: Neuro science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 6-K-PGF1α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 6-K-PGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 6-K-PGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.