ELK0569-48T, Human C1QTNF7(C1q And Tumor Necrosis Factor Related Protein 7) ELISA Kit, 48T

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ELK0569-96T, Human C1QTNF7(C1q And Tumor Necrosis Factor Related Protein 7) ELISA Kit, 96T

2.963,10 RON

Human C1QTNF7(C1q And Tumor Necrosis Factor Related Protein 7) ELISA Kit

SKU
ELK0569-96T

Alternative Names: CTRP7

Species: Human

Assay Type: Sandwich

Sensitivity: 1.36 ng/mL

Standard: 160 ng/mL

Detection range: 2.5-160 ng/mL

Sample type: Serum, plasma, tissue homogenates

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1QTNF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1QTNF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1QTNF7 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CTRP7

Species: Human

Assay Type: Sandwich

Sensitivity: 1.36 ng/mL

Standard: 160 ng/mL

Detection range: 2.5-160 ng/mL

Sample type: Serum, plasma, tissue homogenates

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1QTNF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1QTNF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1QTNF7 in the samples is then determined by comparing the OD of the samples to the standard curve.