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ELK0565-96T, GFAP(Glial Fibrillary Acidic Protein) ELISA Kit, 96T
2.963,10 RON
GFAP(Glial Fibrillary Acidic Protein) ELISA Kit
SKU
ELK0565-96T
Alternative Names: Intermediate Filament Protein
Species: General
Assay Type: Sandwich
Sensitivity: 0.68 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Infection immunity;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GFAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GFAP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Intermediate Filament Protein Species: General Assay Type: Sandwich Sensitivity: 0.68 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Infection immunity;Neuro science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GFAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GFAP in the samples is then determined by comparing the OD of the samples to the standard curve. |
