ELK0543-48T, Chicken OCLN(Occludin) ELISA Kit, 48T

ELK0543-48T, Chicken OCLN(Occludin) ELISA Kit, 48T

ELK0544-48T, Chicken TJP1(Tight Junction Protein 1) ELISA Kit, 48T

ELK0544-48T, Chicken TJP1(Tight Junction Protein 1) ELISA Kit, 48T

ELK0543-96T, Chicken OCLN(Occludin) ELISA Kit, 96T

2.963,10 RON

Chicken OCLN(Occludin) ELISA Kit

SKU
ELK0543-96T

Alternative Names: Tight Junction Protein Occludin TM4 Minus

Species: Chicken

Assay Type: Sandwich

Sensitivity: 31.2 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken OCLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken OCLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken OCLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken OCLN in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Tight Junction Protein Occludin TM4 Minus

Species: Chicken

Assay Type: Sandwich

Sensitivity: 31.2 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken OCLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken OCLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken OCLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken OCLN in the samples is then determined by comparing the OD of the samples to the standard curve.