ELK051ES-48T, EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit, 48T

Alternative Names: 25 Hydroxy Vitamin D;

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 7.7 ng/mL

Standard: 5000 ng/mL

Detection range: 78.13-5000 ng/mL

Sample type: Serum, plasma

Assay length: 1.5h

Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.